Coding
Part:BBa_K642006:Design
Designed by: Wilson Lam, Matthew Orton Group: iGEM11_uOttawa (2011-09-23)
cI repressor tagged with yBFP and a VP16 activation domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 711
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Both cI lambda and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with BFP and the VP16 activation domain.
Source
The cI lambda repressor was PCR amplified from a plasmid source of a biobrick (BBa_K105004) that was submitted by iGEM08_ESBS-Strasbourg. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis. The VP16 activation domain was PCR amplified from a plasmid used by our lab called pDVA38.