Coding

Part:BBa_K642006:Design

Designed by: Wilson Lam, Matthew Orton   Group: iGEM11_uOttawaCA   (2011-09-23)

cI repressor tagged with yBFP and a VP16 activation domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Both cI lambda and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with BFP and the VP16 activation domain.

Source

The cI lambda repressor was PCR amplified from a plasmid source of a biobrick (BBa_K105004) that was submitted by iGEM08_ESBS-Strasbourg. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis. The VP16 activation domain was PCR amplified from a plasmid used by our lab called pDVA38.

References